Direct transposition of native DNA for sensitive multimodal ...
2024年5月9日 · In SAMOSA-Tag, nuclei are treated in situ with the non-specific EcoGII methyltransferase, which mediates N6-deoxyadenosine methylation (m 6 dA), and tagmented using hairpin-loaded Tn5 under ...
Efficient low-cost chromatin profiling with CUT&Tag - Nature
2020年9月10日 · The protein A-Tn5 (pA-Tn5) transposome, when loaded with mosaic end adapters and activated by magnesium ions, integrates the adapters into nearby DNA to create fragments that are amplifiable to...
Nano-CUT&Tag for multimodal chromatin profiling at single-cell ...
2023年12月21日 · Nano-CT implements a new set of secondary nanobody-Tn5 fusion proteins to direct barcoded tagmentation by Tn5 transposase to genomic targets labeled by primary antibodies raised in different...
OpenTn5: Open-Source Resource for Robust and Scalable Tn5 …
2024年7月11日 · Tn5 is a bacterial cut-and-paste transposase, which can simultaneously fragment target DNA and append its ends with custom DNA adapter sequences in a process referred to as tagmentation .
Enhancing sensitivity and versatility of Tn5-based single cell omics
2023年11月22日 · Tn5 transposase is a crucial enzyme for the analysis of chromatin features in single cells as it can simultaneously fragment target DNA across the genome and integrate adapter sequences of choice (Adey, 2021). This “cut-and-paste” reaction is referred to as tagmentation and has been initially used for preparation of DNA sequencing library.
Tn5 DNA Transposase in Multi-Omics Research - MDPI
Addition of a dimeric complex of Tn5 transposase loaded with MEDS adapters (mosaic ends (ME, red) and ssDNA overhangs (blue and green)) to dsDNA results in a DNA double-strand cut and MEDS attachment to the DNA leaving 9-nucleotide gaps (9n) …
Efficient low-cost chromatin profiling with CUT&Tag
We recently introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling strategy in which antibodies are bound to chromatin proteins in situ in permeabilized nuclei. These antibodies are then used to tether the cut-a. … more. Figures (0) & Videos (0) Fig. 1 : Steps in antibody-targeted chromatin profiling strategies.
Tn5 Transposase Applied in Genomics Research - MDPI
2020年11月6日 · Tn5 Transposase Applied in Genomics Research. by. Niannian Li. 1,†, Kairang Jin. 1,†, Yanmin Bai. 2,†, Haifeng Fu. 1, Lin Liu. 1,* and. Bin Liu. 3,4,* 1. College of Life Sciences, Nankai University, Tianjin 300071, China. 2. State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400700, China. 3.
Biochemical analysis of nucleosome targeting by Tn5 transposase
2019年8月14日 · We found that Tn5 transposase preferentially targets near the entry–exit DNA regions within the nucleosome. Tn5 transposase minimally cleaved the dinucleosome without a linker DNA, indicating that the linker DNA between two nucleosomes is important for the Tn5 transposase activity.
Large-Scale Low-Cost NGS Library Preparation Using a Robust Tn5 ...
Tn5 enables fast, robust, and highly efficient processing of limited input material while scaling to the parallel processing of hundreds of samples. Here, we present a robust Tn5 transposase purification strategy based on an N-terminal His 6 -Sumo3 tag.