Macrophage phagocytosis assay with reconstituted target …
2020年6月19日 · To study how phagocytosis is affected by specific changes to the target surface, we developed an in vitro assay based on reconstituted membrane-coated target particles to which known molecules...
Measuring the phagocytic activity of cells - PubMed
These criteria will include choice of phagocytic cell type, selection, and method of labeling of particle for monitoring internalization, targeting of particles to specific receptors, and quantification of ingestion either at the single cell or at the population level.
Vybrant™ Phagocytosis Assay Kit - Thermo Fisher Scientific
The Vybrant Phagocytosis Assay Kit is designed to provide a model system for quantitating the effects of drugs or other environmental factors on phagocytic function. In this assay, cells are incubated with green-fluorescent.
In-Depth Characterization of Monocyte-Derived Macrophages …
2019年2月13日 · Here we developed a novel mass cytometry-based phagocytosis assay that enables the simultaneous detection of phagocytic events in combination with up to 40 other protein markers.
Phagocytosis | Thermo Fisher Scientific - US
Probes for studying phagocytosis include BioParticles indicators—bacteria and yeast labeled with fluorescent dyes. Tracking phagocytosis using a quench/wash-based assay can report on simple uptake, or a pH indicator can be used monitor stages in the pathway.
Use of Flow Cytometry to Evaluate Phagocytosis of - Frontiers
2021年2月18日 · Our rapid assay to monitor phagocytosis can be used to study neutrophil deficiencies and bacterial evasion, but also provides a powerful tool to assess the opsonic capacity of antibodies, either in the context of natural immune responses or immune therapies.
A quantitative method for measuring innate phagocytosis by …
2013年10月16日 · Phagocytosis is central to immunity however a rapid and standardized method is much needed for quantitative assessment of the phagocytic process. We describe a real-time flow cytometric method to quantitate the phagocytosis of fluorescent latex beads by human monocytes in serum-free conditions.
Analysis of macrophage phagocytosis: quantitative assays of …
Phagocytosis of invading pathogens by macrophages represents a fundamental component of the innate immune system. In this chapter, we describe protocols designed for high-throughput analysis of phagosome formation and maturation using latex beads as model phagocytic targets.
Quantitative Phagocytosis Assays in Primary and Cultured
This chapter describes methods to induce and quantify phagocytosis in primary macrophages and in myeloid cell lines. To this end, we initially detail the isolation of primary human monocytes and their differentiation into macrophages.
Macrophage phagocytosis can be triggered by diverse receptor-ligand interactions to clear pathogens and dead cells from a host. Many ways of assaying phagocytosis exist that utilize a variety...