白血病中的转录调控网络 - 知乎 - 知乎专栏
为了研究AML中潜在的RBP依赖性，作者使用II型CRISPR系统，高通量地鉴别癌症存活所必须的基因。. 作者整合了AML患者的CRISPR结构域筛选与转录组分析，鉴别与AML患者生存率相关且在AML中表达失调的RBPs。发现所鉴定的编码21个RBPs的基因在AML筛选中很多都是高度损耗 ...
Discovery of cancer drug targets by CRISPR-Cas9 screening of
2015年5月11日 · We employed a murine MLL-AF9/Nras G12D acute myeloid leukemia cell line (RN2), which has been used extensively to identify genetic dependencies and therapeutic targets by RNA interference (RNAi...
Systematic evaluation of AML-associated antigens identifies anti …
2023年10月23日 · Abdel-Wahab and colleagues perform immunophenotyping of AML cells to identify AML-specific surface antigens and show that the RNA helicase U5 snRNP200 represents a therapeutic target for antibody...
High-performance CRISPR-Cas12a genome editing for ... - Nature
2020年7月13日 · We performed the opAsCas12a- and SpCas9-based screens in a murine Mll-Af9/Nras G12D acute myeloid leukemia cell line (RN2), which has previously been used to identify epigenetic dependencies...
A membrane-associated MHC-I inhibitory axis for cancer ... - Cell …
2023年8月8日 · Notably, we identified a membrane-associated inhibitory axis of MHC-I surface expression that involves sushi domain containing 6 (SUSD6) and transmembrane protein 127 (TMEM127), which together recruit the E3 ubiquitin ligase WWP2 for …
Targeting an RNA-Binding Protein Network in Acute Myeloid ... - Cell …
2019年3月18日 · We designed sgRNAs targeting the RRM domains of Rbm39 and confirmed knockout of Rbm39 protein in RN2 cells (Figure S2I); subsequently sgRNA-expressing RN2 cells were injected intravenously into sublethally irradiated recipient mice.
Role of SWI/SNF in acute leukemia maintenance and enhancer-mediated
2013年12月12日 · Murine MLL-AF9/Nras G12D AML cells (RN2 cell line) transduced with shRNAs targeting Brg1 were rapidly outcompeted by untransduced cells within 6 d in culture—effects nearly as strong as a validated shRNA targeting the BET protein Brd4, which is a known requirement in this model (Fig. 1A; Zuber et al. 2011c).
Cell
2023年8月31日 · RN2-OVA cells were transduced with the indicated sgRNAs and shRNAs, sorted and expanded for 8 days followed by co-culturing with OT-I C8 + T cells. Briefly, 50,000 sgRNA-transduced RN2-OVA cells were seeded into each well of a 96-well U-bottom plate together with 4,000 CD8 + T cells freshly isolated from the lymph nodes of OT-I mice or C57BL/6J ...
BET Bromodomain Inhibition Suppresses the Function of …
2015年6月18日 · Remarkably, exposing RN2 cells to C646 for 2 hr led to a genome-wide reduction of BRD4 occupancy (Figures 3 D and 3E). This effect occurred with about equal potency at enhancer and promoter regions, with slightly greater effects at super-enhancers (Figures 3 D, 3E, and S3 A).
Histone H2B ubiquitin ligase RNF20 is required for MLL ... - PNAS
2013年1月18日 · Two independent shRNAs targeting Rnf20 were induced in RN2 cells with dox for 4 d followed by RNA sequencing analysis. We ranked all expressed genes based on their fold change in mRNA level following Rnf20 suppression, which, importantly, validated Rnf20 itself as among the most down-regulated genes ( Fig. 3 A ).