Purpose : To transfer mouse 2-8 cell embryos into 0.5 day pseudopregnant recipient females for any of several procedures; embryo transfer rederivation, subsequent to in vitro fertilization, and following pronuclear injections and cryopreservation recovery.
A Technique for Facile and Precise Transfer of Mouse Embryos
2012年6月12日 · In this study, two techniques of embryo transfer in mice were compared. In conventional technique the oviduct wall was punctured with a 30-gauge needle and the loaded Pasteur pipette with embryos and medium was inserted into the hole.
The Manual for Lab Mouse - 熊本大学
2013年1月10日 · Embryo Transfer into the Oviduct In our laboratory, we transfer 2-cell embryos through the wall of the oviduct of pseudopregnant recipients. This procedure is much easier and simpler to conduct than the conventional embryo transfer procedure, and is therefore suitable for inexperienced users.
Embryo Transfer Surgery - SpringerLink
2019年9月12日 · Embryo transfer surgery is an essential step in the process of generating gene-modified mice, regardless of whether the embryos were modified by DNA, RNA CRISPR components, or embryonic stem cells, or whether they were microinjected or electroporated.
The first oviduct transfer of mouse embryo was reported by Tarkowski 5, and then Whittingham demonstrated a widely-used method in rat and mouse that turned into the routine procedures for transfer of manipulated embryos 6. In convention-al oviduct and uterus embryo transfer normally a puncture hole or small incision is first made in the
Reproductive Performance after Unilateral or Bilateral Oviduct Transfer ...
In contemporary mouse facilities, embryo transfer (ET) is indispensable for rederivation purposes. ET can be performed unilaterally or bilaterally after general anesthesia. Unilateral ET involves an incision of the skin along the dorsal midline followed by another in the dorsal abdominal wall over the ovary; alternatively, incisions can be made ...
Utero-tubal Embryo Transfer and Vasectomy in the Mouse Model
2014年2月28日 · Murine preimplantation embryos can be transferred to a recipient female either into the oviduct via the ampullae of 0.5 days post coitum (dpc) pseudopregnant recipients (oviduct transfer) 3,4 or into the uterus of 2.5 dpc pseudopregnant recipient (uterine transfer) 5,6 depending on their developmental stage.
C57BL/6J and B6129F1 Embryo Transfer: Unilateral and Bilateral Transfer ...
2020年8月14日 · This procedure consists of the transfer of mouse embryos into the oviduct of a pseudopregnant recipient female in order to obtain live pups. The aim of this study is to further characterize the optimal conditions to perform embryo transfer using wild type strains and particularly the bilateral transfer.
Oviduct Transfer - London Regional Transgenic and Gene …
Air bubbles or blue beads visible inside the oviduct indicate successful transfer. Unclip the Serrefine clamp and remove the mouse from the stereomicroscope. Use blunt fine forceps to pick up the fat pad and place the uterus, oviduct, and ovary back inside the body cavity.
Streamlined ex vivo and in vivo genome editing in mouse ... - Nature
2018年1月29日 · Here we report a novel strategy to generate genetically modified mice by transduction of CRISPR-Cas9 components into pre-implantation mouse embryos via recombinant adeno-associated viruses...