Protein farnesylation: Implications for normal physiology, …
Protein farnesylation is a lipid posttranslational modification required for the cancer-causing activity of proteins such as the GTPase Ras. Although farnesyltransferase inhibitors (FTIs) are in clinical trials, their mechanism of action and the role of protein farnesylation in normal physiology are ill understood.
Protein farnesylation: implications for normal physiology, …
Protein farnesylation was found to be essential for early embryogenesis, dispensable for adult homeostasis, and critical for progression but not initiation of tumorigenesis. Furthermore, Rab geranylgeranyltransferase was identified as a target for some FTIs.
Targeting farnesylation as a novel therapeutic approach in
2022年4月22日 · Activating RAS mutations are found in a subset of fusion-negative rhabdomyosarcoma (RMS), and therapeutic strategies to directly target RAS in these tumors have been investigated, without clinical...
Post-translational modification of KRAS: potential targets for …
2020年10月21日 · In brief, the association of KRAS4B with the plasma membrane depends on its farnesylation and positively charged PBR. For KRAS4A, a combination of cysteine palmitoylation at cysteine and lysine...
Measurement of protein farnesylation and geranylgeranylation
2011年10月27日 · Here we describe protocols to measure the degree of protein prenylation by farnesyl transferase or geranylgeranyl transferase in vitro, in cultured cells and in tumors from animals and humans.
The balance of protein farnesylation and geranylgeranylation …
2020年4月4日 · Protein prenylation is an essential posttranslational modification and includes protein farnesylation and geranylgeranylation using farnesyl diphosphate or geranylgeranyl diphosphate as substrates, respectively.
Tools To Analyze Protein Farnesylation in Cells
2005年9月7日 · Farnesylation of proteins is catalyzed by protein farnesyl transferase (FTase) and is obligatory for the function of the oncoprotein Ras and a variety of other physiologically important proteins.
Tools to analyze protein farnesylation in cells - PubMed
The rapid and selective detection of cellular protein farnesylation status is crucial to understanding both the function of farnesylated proteins and FTase inhibitors. The unnatural FPP analogue 8-anilinogeranyl diphosphate (AGPP, 3b) is an effective alternative substrate for mammalian FTase.
Protein Prenylation: Enzymes, Therapeutics, and Biotechnology ...
Protein prenylation is an irreversible covalent post-translational modification found in all eukaryotic cells, comprising farnesylation and geranylgeranylation. Three prenyltransferase enzymes catalyze this modification.
Protein Farnesylation Is Critical for Maintaining Normal Cell ...
Protein farnesyltransferase (FTase) plays important roles in the growth and differentiation of eukaryotic cells. In this paper, we report the identification of the Schizosaccharomyces pombe gene cpp1+ encoding the β-subunit of FTase.
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