Anion exchange vs cation exchange chromatography
2021年8月4日 · In ion-exchange chromatography, ions are exchanged in two ways. These are anion exchange and cation exchange. A cation is a positively charged ion because it has more protons than electrons and anion is a negatively charged ion because it …
3.4.3. Ion Exchange Chromatography - Chemistry LibreTexts
Generally speaking, a protein will bind to a cation exchange resin if the buffer pH is lower than the isoelectric point (pI) of the protein, and will bind to an anion exchange resin if the pH is higher than the pI.
Ion-Exchange Chromatography - Chemistry LibreTexts
2024年4月16日 · A second sub-category of liquid chromatography is known as ion-exchange chromatography. This technique is used to analyze ionic substances. It is often used for inorganic anions (e.g., chloride, nitrate, and sulfate) and inorganic cations (e.g., lithium, sodium, and …
The 2 Common Types of Ion Exchange Chromatography: Anion vs. Cation
There are several functional groups for anion and cation exchange chromatography, as well as different resolutions. Choosing the appropriate resin and resolution type will depend on your protein of interest and purification purpose.
Ion chromatography - Wikipedia
[5] The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged. The molecule is positively charged because the pH for chromatography is less …
Anion Exchange Chromatography - Bio-Rad
Anion Exchanger vs. Cation Exchanger. The net charge of the molecule(s) of interest determines resin choice. If positively charged molecules are to be immobilized by the column resin, a cation exchange resin is chosen, whereas a positively charged anion exchange resin is chosen if negatively charged molecules are to be captured by the resin.
Introduction to Ion Exchange Chromatography - Bio-Rad
Ion exchange chromatography is a technique for separating compounds based on their net charge. Ion exchange chromatography media contain negatively or positively charged functional groups covalently bound to a solid support, yielding either a …
bind one type of cation but, when presented with a second type of cation, it may displace, and/or exchange with, the first. These resins are called cation-exchange resins. Anion-exchange resins are positively charged and bind and/or exchange negatively charged ions (anions).
Ion exchange chromatography in monoclonal antibodies …
Ion-exchange chromatography (IEX) is a commonly used method for process-scale purification of therapeutic proteins, including monoclonal antibodies. Two main types of IEX are distinguished: anion- and cation-exchange chromatography (AEX and CEX, respectively). In AEX, negatively charged molecules bind to a positively charged stationary phase, whereas in CEX positively …
Positively charged diethylaminoethyl (DEAE) and quaternary ammonium (Q) functional groups, for example, are routinely employed in anion-exchange chromatography, while negatively charged carboxymethyl (CM), sulphomethyl (S), and sulphopropyl (SP) …